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Glocke, P.; Delaporte, K.; Collins, G.; and Sedgley, M.
Micropropagation of juvenile tissue of Eucalyptus erythronema × eucalyptus stricklandii cv. 'urrbrae gem'
In Vitro Cellular & Developmental Biology - Plant
vol. 42, no. 2, 2006, pp. 139-143

Micropropagation via enhanced axillary shoot proliferation was investigated in the ornamental Eucalyptus cv. 'Urrbrae
Gem' using in vitro germinated seedlings and was successfully achieved using woody plant medium (WPM) supplemented
with 2.2mM benzylaminopurine, 1.0 mM a-naphthaleneacetic acid, and 1.5 mM gibberellic acid (GA3), gelled with 5 g l21
Phytagelw. Shoot proliferation was greater on WPM and QL media with GA3 compared to B5, AP, and TK media with or
without GA3. GA3 was required for shoot elongation as the internodes were otherwise very short and unsuitable for
multiplication or root initiation. Root initiation was improved using (1/2)WPM supplemented with 20mM indole-3-butyric
acid (IBA) over a 7 d pulse, followed by subculture to IBA-free medium, compared to placing shoots on low levels of IBA
for 4-6 wk. Plantlets were successfully hardened off to the natural environment via a fogger at 67% relative humidity at
218C for 3 d and continued to thrive as potted plants. This is the first report of successful micropropagation in an
ornamental eucalypt (subgenus Symphyomyrtus) from seedling explants.
Key words: eucalypts rooting; hardening off; media optimization; ornamental.